1、

This procedure using antimitotic medium combining with serum free medium could eliminate fibroblasts effectively, injure less Schwann cells, and yield up to 98% purified Schwann cells.

这种抗有丝分裂法结合无血清培养法,既能有效去除成纤维细胞,又能减少对SC的损伤,获得的SC纯净度可达98%。

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2、

BBG low, medium and high dose groups compared with model group, serum calcium levels increased, alkaline phosphatase decreased with the model group, in which middle and high dose groups were significantly different ( P0.05, P 0.01).

温肾止痛颗粒低、中、高剂量组血钙含量与模型组相比增加,碱性磷酸酶含量与模型组比较有所降低,其中中、高剂量组具有显著差异(P0.05,P0.01)。

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3、

Conclusion: The serum-containing medium for culture of CHO cells can be completely substituted by serum-free media.

结论: 无血清培养基可以完全取代含血清培养基用于培养CHO细胞.

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4、

Methods: Cell culture in serum-free medium , indirect immunofluorescence cytochemistry were used.

方法: 采用无血清细胞培养技术, 间接免疫细胞化学染色法.

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5、

Extensive application in deposit culture medium, serum, medicine as well as microorganism training and environmental testing ect.

广泛用于储藏培养基 、 血清 、 药品以及微生物培养、环境试验等.

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6、

CONCLUSION: The keratinocytes growed best in serum-free medium supplemented with EGF and BPE.

结论: 细胞在加入表皮生长因子和促进增殖的垂体提取物的无血清培养基中增殖良好.

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7、

Results There were 50% survival rate of the hepatocytes in the 10th day cultured in high density containing 20% inactivated allograph male rat serum, 10U/ ml insulin culture medium, high sugar DMEM and they still remained 20% survival rate in the 14th day.

结果含20%同种异体雄性小白鼠灭活血清、10U/ml胰岛素的高糖型DMEM培养基作高密度培养时,50%的细胞成活率在第10天,第14天时仍有20%的存活率;

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8、

The study of direct differentiation of embryonic stem cell into dopaminergic neuron in the serum-free medium

无血清培养条件下诱导胚胎干细胞向多巴胺能神经元定向分化的实验研究

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9、

Methods Neonate rattus cardiac muscle was digested repeatedly by 0.1% trypsin. The cardiocytes were collected in MEM medium which contains 15% fetal bovine serum, then suspended and filtered. The cardiocytes were placed into CO_2 incubator to incubate after purification of them.

方法0.1%的胰蛋白酶重复消化乳鼠心肌组织多次,收集的细胞用含15%胎牛血清的MEM培养基混悬后过滤,纯化心肌细胞后置CO2培养箱孵育。

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10、

Conclusion: Tracheal membrane epithelium cells can be cultured by serum free medium.

结论:应用无血清培养液可进行气管黏膜纤毛上皮细胞的体外培养。组织块培养法培养的纤毛上皮细胞的分化能力保持较好。

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11、

OEG proliferated quickly by free serum medium, and cell count increased by 16 times of primary culture through 9 days cultivation. Its purity is 85%.

通过无血清纯化培养,OEG增殖迅速,原代培养9天,细胞数目可达16倍以上,纯度可达85%。

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12、

Cell culture: The primary OEG was cultivated with the olfactory bulb of Wistar neonate rats, and then the purification was done by free serum medium.

细胞培养:新生Wistar大鼠嗅球(OB)做OEG原代培养,然后利用无血清培养基添加一些促进生长的微量物质做纯化培养,为OEG提供了合适的生长环境而大量增殖。

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13、

Methods The determination of serum calcium was performed with CPA-mA in strong alkaline medium.

方法在强碱性介质中,用偶氮氯膦-mA(CPA-mA)作显色剂测定血清钙。

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